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| Bruce Greenwood Batman | | |
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| Jensen Ackles Red Hood | | |
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| John DiMaggio Joker | | |
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| Neil Patrick Harris Nightwing | | |
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| Jason Isaacs Ra's Al Ghul | | |
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| Wade Williams Black Mask | | |
W pozostałych rolach: Vincent Martella (Robin/Jason Todd), Alexander Martella (młody Robin/Jason Todd)
Carlos Alazraqui (Chi Chi),
Robert Clotworthy (Leon),
Gary Cole (Bobo),
Brian George (asystent Ra'sa),
Kelly Hu (pani Li),
Phil LaMarr (Rick),
Jim Piddock (Alfred),
Kevin Michael Richardson (Tyler),
Andrea Romano (reporter #1),
Dwight Schultz (Freddie),
Fred Tatasciore (najemnik #1),
Keri Tombazian (reporter #2),
Bruce Timm (Riddler),
Michael Villani (reporter #3).